Fig. 7
DNMT3B/miR-451a/EPHA2/PI3K/AKT axis modulates BCa cell growth in vivo. T24 cells (2 × 106 cells/mL) with stable poor expression of DNMT3B or miR-451a were injected into the right armpit of NOD/SCID mice. a, from the fifth day, the growth rate of T24 cells was evaluated by measuring the volume every 5 days in vivo; b, tumor weight; c, KI67 staining intensity in tumors evaluated by immunohistochemistry; d, EPHA2 staining intensity in tumors evaluated by immunohistochemistry; e, phos-PI3K staining intensity in tumors evaluated by immunohistochemistry; f, phos-AKT1 staining intensity in tumors evaluated by immunohistochemistry. Data are represented as the means ± SD from three independent assays. Statistically significant differences were calculated using one-way or two-way ANOVA, followed by Tukey’s multiple comparison test. **p < 0.01 vs mice injected with cells transfected with sh-NC or sh-DNMT3B + inhibitor NC