Fig. 5

The PI3K/AKT signaling is blocked by DNMT3B knockdown. T24 and 5637 cells were treated with shDNMT3B alone or in the presence of miR-451a inhibitor. a, the extents of PI3K and AKT phosphorylation in T24 cells determined by western blot (full-length blots/gels are presented in Supplementary Figure 4A-C); b, the extent of PI3K and AKT phosphorylation in 5637 cells determined by western blot (full-length blots/gels are presented in Supplementary Figure 5A-C). Statistically significant differences were calculated using one-way or two-way ANOVA, followed by Tukey’s multiple comparison test. **p < 0.01 vs sh-NC or sh-DNMT3B + inhibitor NC treatment