Fig. 4

miR-451a targets EPHA2 to regulate the PI3K/AKT signaling. a, the targeting mRNAs of miR-451a predicted by StarBase and analyzed using a DAVID 6.8 online bioinformatics analysis tool. Bubble plots showed 14 signaling pathways; b, gene distribution in the PI3K/AKT signaling in the KEGG database; c, EPHA2 mRNA expression in BCa cell lines T24, 5637, TCCSUP, UM-UC3 and SV-HUC-1 cells determined by RT-qPCR; d, EPHA2 protein expression in BCa cell lines T24, 5637, TCCSUP, UM-UC3 and SV-HUC-1 cells determined by western blot (full-length blots/gels are presented in Supplementary Figure 2A, B); e, EPHA2 mRNA expression in BCa cell lines after transfection determined by RT-qPCR; f, EPHA2 protein expression in BCa cell lines after transfection determined by western blot (full-length blots/gels are presented in Supplementary Figure 3A-D); g, the binding relationship between miR-451a and EPHA2 verified by dual-luciferase assays. Statistically significant differences were calculated using one-way or two-way ANOVA, followed by Tukey’s multiple comparison test. **p < 0.01 vs sh-NC or sh-DNMT3B + inhibitor NC treatment, ***p < 0.001 vs SV-HUC-1 cells