Fig. 3

Overexpression of miR-451a or knockdown of DNMT3B inhibits the aggressiveness of BCa cells in vitro. BCa cells were transfected with shDNMT3B alone or with miR-451a inhibitor. a, miR-451a expression in BCa cell lines determined by RT-qPCR; b, the number of T24 and 5637 cells at the S phase determined by EdU staining where red represents the replicating DNA stained by EdU and blue represents the nucleus stained by DAPI; c, apoptosis in T24 and 5637 cells detected by flow cytometric analysis; d, the formation of apoptotic bodies in T24 cells and 5637 cells determined by TUNEL staining where green represents apoptotic bodies stained by TUNEL and blue represents nuclei stained by DAPI; e, number of migratory T24 and 5637 cells was detected by Transwell assays; f, number of invasive T24 and 5637 cells was detected by Transwell assays. Data are represented as the means ± SD from three independent assays. Statistically significant differences were calculated using one-way or two-way ANOVA, followed by Tukey’s multiple comparison test. **p < 0.01 vs sh-NC or sh-DNMT3B + inhibitor NC treatment