Fig. 5
From: Ovarian cancer cells direct monocyte differentiation through a non-canonical pathway
HGSOC-derived TGFα drives the generation of macrophages. a, b Monocytes were co-cultured with HGSOC cell line OVCAR5 +/− vehicle control (Veh.) or inhibitors against pathways related to the genes of interest: 10 μM DAPT, 5 μM GL, 20 ng/mL anti-AMH, 10 μg/mL mAb225. a CD68+ cells per field of view and (b) CD163+ cells per field of view. Data expressed as average ± SD, n = 3 microdevices per condition; *p < 0.05 vs. Veh. by one-way ANOVA with Dunnett multiple comparison correction. c, d Monocytes were co-cultured with HGSOC cell lines OVCAR5, OVCAR5LIF−/−, or OVCAR5TGF −/−. c CD68+ cells per field of view and (d) CD163+ cells per field of view. Data expressed as average ± SD, n = 3 microdevices per condition; *p < 0.05 vs. OVCAR5 by one-way ANOVA with Tukey multiple comparison correction