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Fig. 8 | BMC Cancer

Fig. 8

From: Identification of ALDH3A2 as a novel prognostic biomarker in gastric adenocarcinoma using integrated bioinformatics analysis

Fig. 8

The expresasion of ALDH3A2 was negatively correlated with PDCD1, PDCD1LG2, and CTLA-4. a The negative correlation between ALDH3A2 and PDCD1 by qPCR; b The negative correlation between ALDH3A2 and CTLA-4 by qPCR; c The negative correlation between ALDH3A2 and PDCD1LG2 by qPCR; d qPCR of ALDH3A2 mRNA expression in GES-1, SGC-7901, MKN45, HGC-27, MGC-803 and AGS cell lines; e qPCR of indicated cells transfected with ALDH3A2-RNAi-vector, ALDH3A2-RNAi; f Western blotting of indicated cells transfected with ALDH3A2-RNAi-vector, ALDH3A2-RNAi; g qPCR revealed that downregulation of endogenous ALDH3A2 significantly decreased the mRNA level of PDCD1, CTLA-4 and PDCD1LG2 in HGC-27 cell lines; h qPCR revealed that downregulation of endogenous ALDH3A2 significantly decreased the mRNA level of PDCD1, CTLA-4 and PDCD1LG2 in MGC-803 cell lines; i-k Western blotting revealed that downregulation of endogenous ALDH3A2 significantly decreased the protein level of PDCD1, CTLA-4 and PDCD1LG2 in indicated cell lines

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BMC Cancer

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