Fig. 4

Effect of UbcH10 and KIAA0101 gene knockdown on NSCLC cell SAC components, cell cycle and proliferation. a 72 h after lentivirus infection, UbcH10 and KIAA0101 and SAC components BubR1, Mad2 CyclinB, CDC25(72 kDa), CDK1(34 kDa) and CDK7(38 kDa) proteins were detected in SK-MES-1 cells. Scanned target protein bands are shown and the y-coordinate of bar chart represents the relative radio optical density values of proteins to β-actin. b Cell cycle analysis of SK-MES-1 cells 72 h after lentivirus infection. c The proliferation of A549 and SK-MES-1 cells 72 h after lentivirus infection were determined by CCK-8 assay. The x-coordinate represents the cell grouping and the y-coordinate represents the absorbance at 450 nm which has a strict linear relationship with cell viability. Three biological replicates of all experiments were performed, and the data are expressed as the mean ± SD, **p < 0.01 and *p < 0.05 vs cell group