Fig. 3

Lentivirus-mediated gene intervention in SK-MES-1 cells: a Detection of the lentiviral infection efficiency of SK-MES-1 cells: 72 h after infection with the recombinant virus (MOI = 10); the effectiveness was estimated by comparing the number of cells with fluorescent marker GFP expression (right) with the number of cells in the field of visible light (left) in the same field of view; b the relative content of UbcH10 and KIAA0101 mRNA in each group was detected 72 h after lentivirus infection with d β-actin used as the reference gene, and the difference between groups was calculated by the 2^-ΔΔCt method; and c UbcH10 and KIAA0101 expression was detected 72 h after virus infection. Three biological replicates of all experiments were performed, and the data are expressed as the mean ± SD, **p < 0.01 and *p < 0.05 vs cell group