Fig. 3

ProEGCG induced apoptosis in RL95–2 and AN3 CA cells in a time-dependent manner. Cells were treated with increasing concentrations of EGCG and ProEGCG (a) RL95–2 cells and (b) AN3 CA cells for 48 h and (c) RL95–2 cells and (d) AN3 CA cells for 72 h. Apoptotic cells were quantitatively analyzed by flow cytometry with annexin V-FITC and propidium iodide staining. e Lysates from cells treated with ProEGCG and EGCG were assayed for PARP and caspase-3 cleavage by Western blotting. GAPDH was used as a loading control. Data are presented as mean ± S.E.M. Significant differences from control: *P < 0.05 and **P < 0.01