Fig. 5

Increased cytotoxic T cell infiltration in IKFM tumors in established tumor and implantation models. Harvested TBR5 omental tumors from the established tumor model (a-d) and implantation model (e-h) were stained for the pan T cell marker CD3 (red) and the cytotoxic T cell marker CD8 (green) for immunofluorescence analysis. DAPI-stained nuclei are in blue. a, e) Representative 40x immunofluorescent images. Semi-automated counts of total CD3 positive cells (b & f) and total CD8 positive cells (c & g) were performed, with the ratio of CD8 to CD3 positive cells shown in d & h. Counts were quantified from 5 high-power fields and represent percentage of cell subtype relative to total cellularity in each field. Values are mean + SEM of n = 3 for each group; P-values are from the Mann-Whitney test. Figure 5a and e were generated with Adobe Photoshop CC 2018 (Version 19.0.1). Figure 5b-d and f-h were generated with GraphPad Prism (Version 8: La Jolla, California, USA)