Fig. 2

Effects of NF-κB activation in established TBR5 tumors in IKFM mice. FVB IKFM and control mice injected with TBR5 cells were treated with 1 g/L dox from 7 to 21 days post-tumor cell injection (in red), with a two-day break from days 12–14. a Schematic of experimental design. b Harvested omental tumor weight and c ascites volume at sacrifice. Values are mean + SEM; n = 10 per group. d Representative low-power 4x magnification H&E images of each group, with e showing corresponding high-power 20x images of the boxed areas. f Representative images of immunofluorescent staining of harvested TBR5 omental tumors for F4/80 (macrophages; green), arginase-1 (M2 macrophage marker; Arg-1; red), and DAPI (nuclei; blue). Semi-automated counts of g percent of F4/80 positive cells per high-power field (HPF), h percent of Arg-1 positive cells per HPF, and i the ratio of Arg-1⁺ to F4/80⁺ cells. Counts were quantified from 5 high-power fields from each representative mouse sample and represent percentage of cell subtype relative to total cellularity in each field. Values are mean + SEM of n = 3 for each group; P-values are from the Mann-Whitney test. Figure 2a was generated with Microsoft PowerPoint 2016 (Version 16.16.26); Fig. 2b, c, and g-i were generated with GraphPad Prism (Version 8: La Jolla, California, USA); and Fig. 2f was generated with Adobe Photoshop CC 2018 (Version 19.0.1)