Fig. 4

Hierarchical cluster of canonical pathways. A totally drug-sensitive LK-2 cell line and a drug-resistant RERF-LC-AI cell line were treated with or without TXT and Ramucirumab in single and combination treatment settings, and then subjected to RNA-seq analysis. Using the gene expression data, genes highly correlate in terms of expression levels of each target gene were assessed, and then hierarchical clustering of the canonical pathways was performed using “hclust” from the stats package in R software. Among the 235 target pathways, 50 representative canonical pathways are listed in this figure and the other data are shown in Additional file 5