Fig. 3

Wound healing assay in HCC cells. The cell layer was scratched with a pipette tip. Cells were then treated with acteoside (A01; 100 or 500 μM) and sorafenib (sora; 10 μM) alone or in combination. Cell migration was visualized at the indicated time point (0, 10 and 24 h) under an inverted light microscope at a magnification of × 100. Data are expressed as mean ± SD from three independent experiments. Scale bar, 500 μm. *,p < 0.05, **,p < 0.01 vs the control group or otherwise as specified