Analytical performance evaluation of a commercial next generation sequencing liquid biopsy platform using plasma ctDNA, reference standards, and synthetic serial dilution samples derived from normal plasma

Table 2 Sample QC Metrics. Data in Table was generated by AVENIO OAS

Panel		Input DNA Mass (ng)	# Read Pairs	Sequencing Depth Median	Unique Depth Median	Unique Depth 5’th Percentile	Unique Depth 95’th Percentile	Error Rate	On-Target Rate	Bases Within 10-fold Range of Median	N
AVENIO Expanded Kit	Minimum	10	23,167,961	8146	1046	846	3248	0.000004	54%	98%	28
	Maximum	50	38,724,317	15,142	7423	3258	14,192	0.000064	78%	99%	28
	Average	31	29,508,308	10,054	4632	2182	9686	0.000017	69%	99%	28
AVENIO Targeted Kit	Minimum	11	14,972,469	15,076	1121	721	1855	0.000005	70%	100%	29
	Maximum	50	25,743,805	24,697	8028	5900	17,389	0.000021	81%	100%	29
	Average	28	20,645,107	19,999	3423	2493	5757	0.000010	77%	100%	29
AVENIO Surveillance Kit	Minimum	20	25,469,334	5451	2626	1485	4460	0.000003	26%	100%	10
	Maximum	50	38,331,741	15,096	7857	3336	11,878	0.000072	79%	100%	10
	Average	29	33,150,021	11,223	5041	2393	7432	0.000034	65%	100%	10

ISSN: 1471-2407