Fig. 4

HOXD-AS1 induced FGF2 expression by competitively sponging miR-877-3p in CC cells. a The wild type (in green) and mutant (in red) binding sites between FGF2–3′-UTR and miR-877-3p were obtained from the bioinformatics software TargetScan Human Release 7.2. b Luciferase activity was measured in Hela cells co-transfected with wild type FGF2 3’UTR (FGF2–3′-UTR-wt) or mutant type FGF2 3’UTR (FGF2–3′-UTR-wt) and miR-877-3p using the dual luciferase reporter assay. c qRT-PCR analysis of FGF2 mRNA level following treatment of Hela cells and with miR-877-3p mimics (miR-877-3p-M). d Western blot analysis of FGF2 protein level following treatment of Hela cells with miR-877-3p mimics (miR-877-3p-M). e qRT-PCR analysis of FGF2 mRNA level in Hela cells with HOXD-AS1 knockdown or overexpression (si HOXD-AS1 or 3.1-HOXD-AS1). f Western blot analysis of FGF2 protein level in Hela cells with HOXD-AS1 knockdown or overexpression (si HOXD-AS1 or 3.1-HOXD-AS1). **: P < 0.01, ***: P < 0.001, NS: not significant