Fig. 3

Chromatogram depicting LOD of MAF in Ex19_std and Ex21_std. a: Ex19_std with varying MAF 10%, 5%, 1% & 0.5% from top to bottom in a total DNA concentration of 2.5 ng/μl. b: Chromatogram of Ex19_std with varying MAF 25%, 10% & 5% from top to bottom in a total DNA concentration of 0.25 ng/μl. c: Chromatogram depicting Ex21_std with varying MAF 5%, 0.5%, 0.25%, 0.125% from top to bottom in a total DNA concentration of 2.5 ng/μl. d: Chromatogram of Ex21_std with varying MAF 25%, 10%, 5% & 2.5% from top to bottom for a total DNA concentration of 0.25 ng/μl. Note that the detection limits of Ex19_std & Ex21_std were 1% and 0.25% for 2.5 ng/μl assay and 10% and 5% for a 0.25 ng/μl assay