Fig. 2

PANC-1 cell growth and clustering of proteomic expression during exposure to paclitaxel and birinapant. a Representative model-fitted profiles (solid lines) of PANC-1 cell proliferation measured experimentally (symbols) over 120 h of exposure to vehicle control (blue circles), single-agent BRP (green squares), PTX (red triangles), and BRP/PTX combined (black crosses). Observed cell proliferation is normalized to t = 0 h, and all observations are in triplicate. Dashed lines are cell number after correction for the polyploid cells. b The ratio of observed data to values computed assuming additive drug interaction for the BRP/PTX combinations. White circles represent median values, vertical black bars represent the 2nd to 3rd quartiles, the grey shapes of violins represent histograms of the data, and horizontal bars at the ends of violins show data ranges. c. The clustering of temporal protein expression responses to birinapant/paclitaxel treatment. The k-means clustering algorithm was applied to quantitative proteomics data for PANC-1 cells exposed to vehicle (0.1% DMSO), or 100 nM birinapant and 10 nM paclitaxel, alone or combined, for 6, 24, 48 and 72 h