Fig. 1
In-vitro viability under 3-BrPA treatment and expression of apoptosis and markers of cell cycle in MPNST cell lines. c(3-BrPA) – concentration of 3-BrPA; ∆FUnorm – normalized difference of fluorescence units. a Dose-dependent courses of viability in S462, NSF1, T265, and BRGN cells on agent concentration in a range of 0 to 120 μM. Mean values of normalized differences in FU according to Eq. (1) standard errors of the mean (SEM) are presented. Concentration ranges with significant differences (p < 0.05) in cell viability compared to BRGN cells are marked by horizontal lines and asterisks. b Responses of the cell lines dependent on 3-BrPA concentration. Significant differences in cell viability compared to BRGN cells are highlighted by asterisks. c Correlations between cell viability and agent concentration as nonlinear regression functions. For details of statistics see Additional file 1. d Histopathological and immunohistochemical analysis of untreated T265 (left), NSF1 (middle) and S462 cells (right). Scale bars represent 100 μm in each photograph