Fig. 2

GCN5 protein expression correlates with WDHD1. a GCN5 and WDHD1 protein levels in RPE1 cells. b GCN5 protein levels in RPE1 E7 cells examined by Western blotting after retransfected with Flag-WDHD1. Data were summarized (Right panel). c RPE1 cells were incubated with cycloheximide (CHX) and harvested at the indicated times. The stability of GCN5 was monitored by immunoblotting analyses (left panel). Data are summarized in the right panel. Data from a representative experiment of 3 are shown, *P < 0.05; **P < 0.01. The WB samples for quantitative comparisons on different gels/blots derive from the same experiment and that gels/blots were processed in parallel. Cropping is used for the gels and blots in the main paper and the ‘full-length blots/gels are presented in Supplementary Figure 2