Fig. 2

MiR-876-5p suppressed proliferation and motility in astrocytoma cells (a-b) Nuclear cytoplasm fractionation and FISH were built to judge the subcellular place of SNHG17 in U138 and LN-215 cells. c RNA pull down was used to select out miRNAs could bind to SNHG17. d MiR-876-5p expression was tested in astrocytoma cell lines. e The binding sites between SNHG17 and miR-876-5p were predicted by starBase. f RNA pull down manifested that miR-876-5p could bind to SNHG17. g RIP verified that SNHG17 could bind to Ago2. h MiR-876-5p expression was detected in astrocytoma cells. i Luciferase reporter assays illustrated that SNHG17 could bind to miR-876-5p in both LN-215 and U138 cells. j-k Proliferative capacities were examined in EdU and CCK8 in cells with NC mimics and miR-876-5p mimics. l-m Cell apoptosis was evaluated in different groups by TUNEL assay and flow cytometry analysis. n-o Stemness of cells in different groups was probed via sphere formation assay and RT-qPCR analysis of related genes. p-q Transwell assay examined migration and invasion. ^**P < 0.01