Fig. 5

Knockdown of OTX1 inhibits tumor growth in a xenograft mouse model. a Representative images of day 28 tumors formed in nude mice that had been subcutaneously injected with Hep2 cells transfected with shOTX1–1, shOTX1–2 and SC. b The volumes of tumors dissected from the shOTX1–1 and shOTX1–2 group was lower than the SC group. c The tumor weight were significantly reduced following the injection of shOTX1–1 or shOTX1–2-expressing tumor cells compared with the SC group. d Representative images of day 28 tumors formed in nude mice that had been subcutaneously injected with TU212 cells transfected with shOTX1–1, sOTX1–2 and SC. e The volumes of tumors dissected from the shOTX1–1 and shOTX1–2 group was lower than the SC group. f The tumor weight were significantly reduced following the injection of shOTX1–1 or shOTX1–2-expressing tumor cells compared with the SC group. g, h. Representative HE and ki67 IHC staining image. SC: scrambled shRNA, #1: shOTX1–1, #2: shOTX1–2. **P < 0 .01