Fig. 5
SNHG3 intervention can inhibit the growth of BC cell xenograft tumor in vivo. MCF-7 cells stably SNHG3-siRNA and scramble siRNA were inoculated subcutaneously into BALB/c nude mice at a dose of 5 × 106 per mouse (n = 6 in each group). Tumor growth was measured continuously every 5 days, and 20 days later, tumor growth was monitored every 3 days. At 35 days post-implantation, the mice were euthanized by carbon dioxide asphyxiation. a. Tumor size; b. Tumor weight and representative view of xenografts. Tumor sections were obtained and stained with anti-Notch1, anti-Notch2 and anti-Notch3 antibodies; c. Representative views of Notch1, Notch2 and Notch3-positive tumor cells and quantification of immunohistochemistry. Data are expressed as mean ± standard deviation. One-way ANOVA and Sidak’s multiple comparisons test was used to determine statistical significance, or two-way ANOVA and Tukey’s multiple comparisons test was used, *p < 0.05, **p < 0.01