Fig. 4

Notch signaling pathway activation reversed BC cells proliferation and viability by SNHG3 silencing. MCF-7 stably expressed si-SNHG3–2 was treated with Notch signaling pathway specific activator, Jagged 1. RT-qPCR and Western blot analysis were performed to determine Notch1, Notch2 and Notch3 mRNA (a) and protein (b) levels after Jagged 1 treatment (representative images were shown, full-length gels are presented in Supplementary Figure 3); MCF-7 cells were performed with MTT proliferation assay (c) and EdU staining (d) and colony formation assays (e) to determine Notch signaling pathway activation effectiveness; f. MCF-7 cells migrating from upper Transwell chambers into lower ones, without Matrigel (× 200); g. MCF-7 cells invading from Matrigel-coated upper Transwell chambers into lower ones (× 200); h. Western blot analysis was carried out to determine E-cadherin and N-cadherin protein level (representative images were shown, full-length gels are presented in Supplementary Figure 4). Three independent experiments were performed. Data are expressed as mean ± standard deviation; one-way ANOVA and Sidak’s multiple comparisons test was used to determine statistical significance, or two-way ANOVA and Tukey’s multiple comparisons test was used, *p < 0.05, **p < 0.01