Fig. 6
Angiogenesis on the CAM induced by MDA-MB-231 cells. The non-intervened cancer cells and the cells after cryopreservation were accumulated and transplanted on the relative avascular region of the CAM. a Negative control: 40 μl 1X Phosphate-Buffered Saline. b Representative sample of group 1: 4 × 106 non-intervened cells. c Representative sample of group 2: 8 × 106 non-intervened cells. d Representative sample of group 3: 4 × 106 cryopreserved cells. e Representative sample of group 4: 8 × 106 cryopreserved cells; Scale bar: 2.5 mm. f The reverse side of sample in (e). It was detected numerous radically distributed vasculature and vertically growth into the tissues, which was indicated by the reddishness at the middle of the transplanted sites. Scale bar: 1 mm. Image J software was applied to measure the area of vessels and CAM