Fig. 3

Expression of GATA3 in breast cancer cells before and after cryopreservation. a Cryopreserved and non-intervened ZR-75-1 and MDA-MB-231 cells were immunostained with the GATA3 marker. Cryopreservation led to diminished expression of GATA3 in ZR-75-1 cells. The confocal images showed that GATA3 expression in MDA-MB-231 cells was at an undetectable low level. Scale bar: 50 μm. b The intensity of the fluorescent signal in the cryopreserved cells significantly attenuated compared to the non-intervened samples. Data were analyzed by Student’s t-test and expressed with mean ± SD, ^*p < 0.05. Triplicate samples were included in five independent experiments (n = 5). c Left panel: Cryopreservation downregulated GATA3 expression in ZR-75-1 cells. The blots of the protein expression in MDA-MB-231 cells were not capable of capturing. HSC70 was the loading control. Right panel: The protein expression level equaled the proportion of GATA3/HSC70. Data were calculated using Student’s t-test and expressed with mean ± SD from five independent experiments (n = 5). Significantly different at ^**p < 0.01. The Odyssey Clx (LI-COR) was applied to visualize the bands. Image J software was used to measure the band intensity. These blots were cropped. Full-length blots/gels are presented in Supplementary Figure 1