Fig. 2

PCR products of P1 and P3 containing SNPs in the sequence analysis. a The P1 products of two SNPs, SNP38 (in an intron) and codon 72 (in an exon), of p53 were analyzed by DNA sequencing. Compared with the NCBI PubMed sequence, the sequencing data of the T98G cells presented as CC (single peak, asterisk) at both SNP 38 and codon 72, that is, as homozygous (upper layer). The sequencing data of the U87 cells presented as GG (single peak, asterisk) at both SNP 38 and codon 72, that is, as homozygous (middle layer). The sequencing data of the GBM specimen presented as CG (double peaks, asterisk) at both SNP 38 and codon 72, as heterozygous (lower layer). b Using the same sample and analysis as above, the P3 products of two SNPs, SNP 72 (in an intron) and SNP 92 (in an intron), of p53 were analyzed by DNA sequencing. c The 16-bp duplication polymorphism (rs17878362) of the U87 cells presents at a homozygote (loss of 16-nt). d A few of the GBM specimens presented as heterozygotes of the 16-bp duplication polymorphism resulting in the following sequence presenting a double peak. e BstUI PCR-RFLP analysis of the p53 codon 72 polymorphism. M, DNA ladder. Lane 1, Pro/Pro homozygote is not cleaved by BstUI and yield a single 366-bp band. Lane 2, Arg/Arg homozygote is cleaved by BstUI and yields 215- and 151-bp bands. Lane 3. Arg/Pro heterozygote contains all three bands (366, 215, and 151 bp) following restriction digestion