Fig. 4
From: Therapeutic effect of dual CAR-T targeting PDL1 and MUC16 antigens on ovarian cancer cells in mice
Detection results of target cells overexpressing PDL1 and(or) MUC16. a Detection results of PDL1 antigen on OVCAR3-PDL1-luc cells. OVCAR3-PDL1-luc cells stained with 70ul Steady-Glo for 20 min in the dark. b Detection results of MUC16 antigen on OVCAR3-MUC16-GFP-luc cells. GFP is used to mark the MUC16. c Detection results of MUC16 and PDL1 antigens on OVCAR3-MUC16-GFP-PDL1-luc cells. OVCAR3-MUC16-GFP-PDL1-luc cells stained with 70ul Steady-Glo as the above methods. Control groups are the green peak; Experimental groups are the red peak. The top pictures show the titer test results, and the bottom two show the cell pools and the monoclonal samples