Fig. 1

PT inhibits colony formation. a-b HT-29 c-d SW480 e-f SW620 cells were seeded as single cell at 500 cells/well in 6-well plate. After 4–6 h, PT (5 and 10 μM) was added for 24 h and incubated at 37 °C. After 24 h media containing PT was replaced with fresh complete media and cells were further incubated for 10–12 days for colony at 37 °C. Crystal violet staining was done and colonies were quantified using light microscope and images were captured by Bio-Rad Gel-Doc system. Results are shown as representative of three independent experiment (n = 3). ***p < 0.001 PT (5) vs control; ***p < 0.001 PT (10) vs control