Fig. 2

The inhibition of the mevalonate pathway induces apoptosis in human IGROV-1 and A2780 ovarian cancer cells. a Anti-tumor effects mediated by atorvastatin (ATO), simvastatin (SIM), rosuvastatin (ROSU) or zoledronic acid (ZOL) were assessed by the Caspase 3/7 Glo® assay after treatment of IGROV-1 and A2780 cells for 48 h. Induction of apoptosis was further confirmed using Western blot based detection of cleaved poly (ADP-ribose) polymerase (cPARP) 48 h after treatment of IGROV-1 cells and 24 h after treatment of A2780 cells, respectively. The equal protein loading is shown by detection of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The figures show representative blots which were cropped from original images. Full-length blots are presented in Suppl. Fig. 6. Images were detected using GelCapture 7.0.18 software. b Analysis of the expression of the anti-apoptotic genes survivin (SVV) and B-cell lymphoma 2 (BCL-2) by quantitative real-time-PCR 48 h after treatment of IGROV-1 cells with ATO, SIM, ROSU and ZOL. Data are shown as mean ± SEM of at least three individual experiments. (*p < 0.05; **p < 0.01; ***p < 0.001)