Fig. 1
From: Suppressing Dazl modulates tumorigenicity and stemness in human glioblastoma cells
The expression levels of Dazl in glioblastoma tissues and cell lines. a Dazl expression was examined by immunohistochemical analysis in human glioblastoma tissues and adjacent normal tissues. Strong cytoplasmic expression of Dazl (brown staining) was detected in stage III/IV glioblastoma cells, and the nucleus was stained blue with hematoxylin. Nor: Normal. Images were taken from the inverted microscope (bars = 50 μm, magnification × 200). b The correlation of Dazl expression and glioblastoma grade was analyzed from the Chinese Glioblastoma Genome Atlas (CGGA) data (**P < 0.01, ****P < 0.001). c The correlation of Dazl expression and overall survival of glioblastoma patients was determined from the CGGA data (**P < 0.01). d The lysates of glioblastoma cells from A172, U251, and LN229 cell lines were harvested and examined for Dazl expression, the cell lysates of NHA cell line were used as the negative control (**P < 0.01, ****P < 0.001). e Detection of gene expression by agarose gel electrophoresis after RT-PCR. f Dazl expression in glioblastoma cell lines was detected by western blotting. g Relative Dazl expression was quantified by Image J software using Gapdh as an internal control. (*P < 0.05)