Fig. 4

LINC00992 promoted prostate cancer cell proliferation and migration via elevating GOLM1 expression. a-b GOLM1 mRNA and protein levels in DU145 cells transfected with pcDNA3.1 or pcDNA3.1/GOLM1 were detected via qRT-PCR and western blot. pcDNA3.1 served as the negative control. c The viability of DU145 cells was determined via CCK-8 following transfection of different plasmids. d The proliferation of transfected cells was evaluated via EdU assay. e The apoptosis of transfected cells was monitored via TUNEL assay. Scale bar = 200 μm. f The protein levels of Bax and Bcl-2 in different groups were detected via western blot. g-h The migration of transfected cells was measured via Transwell migration assay (scale bar = 100 μm) and wound healing assay (scale bar = 100 μm). The full-length gels for western blot data in Fig. 4b and f were presented in Supplementary Figure 6. *P < 0.05, **p < 0.01