Fig. 3

LINC00992 regulated the expression of GOLM1, a target of miR-3935. a The expression of eight mRNAs in four prostate cancer cell lines and RWPE-1 cells was detected by qRT-PCR. b GOLM1 was overexpressed in prostate cancer tissues according to GEPIA database. c The mRNA and protein levels of GOLM1 were evaluated in prostate cancer cell lines and RWPE-1 cell line by qRT-PCR and western blot, respectively. d The binding sites between GOLM1 and miR-3935 were predicted via TargetScan. e Luciferase reporter assay presented the inhibited luciferase activity of GOLM1-WT reporter in the presence of miR-3935 mimics not NC-mimics. f-g GOLM1 expression in transfected cells was tested by qRT-PCR and western blot analyses. h The combination of GOLM1 with miR-3935 in the anti-Ago2 group was validated by RIP assay. i-j The mRNA and protein levels of GOLM1 in different groups were examined via qRT-PCR and western blot. The full-length gels for western blot data in Fig. 3c, g and j were presented in Supplementary Figure 5. *P < 0.05, **p < 0.01, ***p < 0.001