Fig. 5

The effect of knock-down of p53 and p53AIP1 genes on apoptosis efficiency induced by ADI. Cells were separately co-transfected by pcDNA4-ADI plasmids with siTP53 or siTP53AIP1. Cell apoptosis rates were detected by flow cytometry after the static cell culture for 48 h. a: Representative images of FACS analysis of annexin V and PI staining of HepG2 and PC3 cells. b: Death ratio summary of FACS analysis from Fig. 5a. c: Fluorescence assay for mitochondrial permeability transition pore (MPTP) from Fig. 3a. d: Immunoblots of ADI, p53 and p53AIP1 protein expression in HepG2 and PC3 cells. C-myc-tag antibody was used to detect c-myc-tag-fused ADI. Full-length blots are presented in Supplementary Fig. S5. e: the relative quantification for protein expressions in PC3 and HepG2 cell lines. Grey scales of protein bands from Fig. 5d were detected by ImageJ 1.52. P values were calculated by comparing siRNA-treated cells with scrRNA-treated cells in the respective cell lines. **P < 0.01; ***P < 0.001