Fig. 4

Molecular mechanism of cell apoptosis induced by arginine deprivation. a: mRNA level detection of some factors related with mitochondria apoptosis pathway by Quantitative RT-PCR in PC3 and HepG2 cells. b: Immunoblot of the factors related with apoptosis pathway in PC3 and HepG2 cells. Full-length blots are presented in Supplementary Fig. S4. c: Fluorescence assay for mitochondrial permeability transition pore (MPTP). d: Activity assay of Caspase 3 through caspase 3 assay kit (Colorimetric) (abcam. ab39401). e: Activity assay of Caspase 9 through caspase 9 assay kit (Colorimetric) (abcam. Ab65608). f/g: The relative quantification for protein expressions in PC3 and HepG2 cell lines. Grey scales of protein bands from Fig. 4b were detected by ImageJ 1.52. P values were calculated by comparing pcDNA4-ADI plasmids-treated cells with pcDNA4 plasmid-treated cells in the respective cell lines. **P < 0.01; ***P < 0.001