Fig. 1From: Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagyApoptosis efficiency induced by ADI expressed in MRC5, HepG2 and PC3 cells. Cells were separately transfected by pcDNA4, pcDNA4-ADI plasmids. Cell apoptosis rates were detected by flow cytometry after the static cell culture for 48 h. a: Representative images of FACS analysis of annexin V and PI staining of MRC5, HepG2 and PC3 cells. b: Death ratio summary of FACS analysis from Fig. 1a. c: Immunoblots of ADI and ASS expression in MRC5, HepG2 and PC3 cells. C-myc-tag antibody was used to detect c-myc-tag-fused ADI. The blot of GAPDH was from the same gel as the blot of ADI. Full-length blots are presented in Supplementary Fig. S1. d: the relative quantification for protein expressions in MRC5, PC3 and HepG2 cell lines. Grey scales of protein bands from Fig. 1c were detected by ImageJ 1.52. P values were calculated by comparing pcDNA4-ADI plasmids-treated cells with pcDNA4 plasmid-treated cells in the respective cell lines. **P < 0.01; ***P < 0.001Back to article page