Fig. 4

IMMUNEPOTENT CRP induces ATP and HMGB1 release in HeLa and MCF-7 cells. Cells were treated with ICRP (HeLa: 1.25 U/mL; MCF-7: 1.5 U/mL) for 24 h, then 100 μL supernatant of each sample was taken to measure a. ATP release through bioluminescence detection or b. HMGB1 release by ELISA. Graphs shown are means (± SD) of triplicates of three independent experiments