Fig. 6

Virus quantification of the combinatorial approach. Virus titer growth curves were performed with H727 and BON-1 tumor cells under the same conditions in presence of everolimus (added at 1 hpi). Previous results from monotherapy (Fig. 4) are shown (solid lines). Interestingly, everolimus did not alter viral replication in any significant way (dotted lines). Plaque forming units (PFU) were determined every 24 h; samples were analyzed in duplicates; experiments were performed twice; one representative result is shown