Fig. 4

The effect of NR2F2 overexpression on EMT in MCF-7. a MCF-7 cells were transfected by NR2F2 overexpressed plasmid and empty vector, the protein expression of NR2F2 was detected by Western blot. b The NR2F2 mRNA expression was detected by real-time PCR. c The protein expression of EMT markers including E-cadherin and vimentin were detected by western blot in cells transfected with empty or NR2F2 overexpressed plasmid (n = 5). d The mRNA expression of EMT markers was detected by quantitive real-time PCR in cells transfected with empty or NR2F2 overexpressed plasmid (n = 5). e and f Plasmid transfected MCF-7 cells were seeded into the 8 μm-pole transwell chamber for 48 h. The number of transmembrane cells was counted (n = 4). *P < 0.05, **P < 0.01 vs. empty