Fig. 3

The effect of insulin on NR2F2 expression in breast cancer cells. a Protein expression of NR2F2 was measured in MDA-MB-231 and MCF-7 cells. b mRNA expression of NR2F2 was measured in MDA-MB-231 and MCF-7 (n = 5). c Dose effect of insulin at concentration of 0, 2, 4, 8, 16 μM for 24 h on NR2F2 protein expression in MDA-MB-231 cells. d Time effect of insulin at 4 μM for 0, 3, 6, 12, 24 h on NR2F2 protein expression in MDA-MB-231 cells. e NR2F2 mRNA expression was measured in MDA-MB-231 after insulin incubation for 24 h. f mRNA expression of NR2F2 in MCF-7 cells was detected after cultured in insulin for 12 and 24 h. g MCF-7 cells were cultured in insulin for 12 and 24 h, NR2F2 protein expression was detected. **P<0.01 vs. control