Fig. 6

Effect of FXR knock down on the synthesis of bone proteins. Scr: scramble, 10 = clone 10, 13 = clone 13. a Western blotting of FXR in MDA-MB-231 cells after exposure to shRNA. Immuno bands are quantified and normalized with β-actin expression. Immunoreactive band intensities were quantified using the software ImageJ®. Full-length blots are presented in supplementary Figure 8b FXR expression and quantification in MDA-MB-231 cells after shRNA. c RUNX2 respective expressions after CDCA treatment in in MDA-MB-231. d OPN respective expressions after CDCA treatment in MDA-MB-231. e Western blotting of FXR in MCF-7 cells after shRNA. Values are quantified and normalized on β-actin expression. Immunoreactive band intensities were quantified using the software ImageJ®. Full-length blots are presented in supplementary Figure 9f FXR expression and quantification in MCF-7 cells after exposure to shRNA. g RUNX2 respective expressions after CDCA treatment in in MCF-7. h OPN respective expressions after CDCA treatment in MCF-7. Clone 10 and 13 showed the lowest expression in MDA-MB-231 and MCF-7 respectively. The decrease of FXR expression caused by shRNA significantly block the induction of RUNX2 and OPN expression reduction after CDCA treatment in clones compared to scrambles. (*** p < 0.001, ** p < 0.01)