Fig. 3
Effects of increased IL-8 levels on hematopoietic stem cells. a Conditioned media was collected from 24-h cultures of healthy hBMSCs grown in mesenchymal stem cell growth medium. Subsequently, 2.5 × 106 hHSCs were cultured for 24 h in conditioned media in the presence n = 12) and absence (n = 12) of IL-8 (100 ng/mL). The numbers of total, CD34+, and CD34+/CD45- cells were significantly higher in the hHSCs cultured in the presence of IL-8, compared to those in cells cultured without IL-8 (p = 0.014, p = 0.020, and p = 0.039, respectively). b The relative expression of CXCR2, mTOR, and c-MYC increased at 1 h after IL-8 treatment. The expression of CXCR2 returned to normal after 6 h of IL-8 treatment, and the expression of mTOR gradually decreased at 6 and 24 h after IL-8 treatment. In the case of c-MYC, the increased expression lasted up to 24 h. Each experiment was repeated thrice. Note: *** p < 0.001; ** p < 0.01; * p < 0.05. Note: Values are reported as the median with range (A) and the mean ± SEM (B). Abbreviations: hBMSCs, human bone marrow stromal cells; hHSC, human hematopoietic stem cell