Fig. 5
From: DNA methylation signatures of Prostate Cancer in peripheral T-cells
Identification and validation of cg05133736 as a DNA methylation marker using Illumina EPIC (850 K), amplicon sequencing (using MiSeq system) and pyrosequencing systems. a-b Percentage methylation of T cell DNA of each sample at cg05133736 plotted as; a Ctrl (Control, Blue) and PCa (PCa, Red); b Ctrl (Control, Blue), LGPCa (Low Gleason PCa, Red) and HGPCa (High Gleason PCa, Green). Statistical significance was obtained in GraphPad Prism and are represented by asterisks (*P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001). c Correlation graphs between Illumina EPIC (850 K), amplicon sequencing and pyrosequencing. d ROC curve showing the sensitivity and specificity of cg05133736 for detection of PCa