Fig. 2

Negative expression of CCR6 and CCR8 in OSCC. a Representative images of CCR6 and CCR8 staining in OSCC tissues and adjacent healthy tissues (magnification 100×). b and c Results of western blot showed that CCR6 and CCR8 were rarely expressed in OSCC tissues, adjacent healthy tissues, OSCC cells, and HOK cells. The full-length blots are presented in Supplementary Fig. S2. d and e Immunofluorescence staining of CCR6 and CCR8 (red) and nuclei (blue) in oral cancer cells (HSC-6, CAL27 and SCC9) and HOK cells (magnification 200×)