Fig. 3

Apoptosis and immunogenic cell death of the CT26 cells treated with curcumin, resveratrol and mEHT. (a) CT26 cells were treated with 37 °C (37), water bath (42w), mEHT (42 m), 37 °C (37) + curcumin (C, 20 μM) and resveratrol (R, 25 μM), water bath + CR (42w + CR), and mEHT + CR (42 m + CR) for 3 or (b) 24 h. The apoptotic effects of these treatments were measured by annexin V/propidium iodide staining and flow cytometry. The results were represented the mean ± S.D. of three independent experiments. *P < 0.05, ***P < 0.001. (c) The expressions of HSP70 and caspase 3 were analyzed by western blot after CT26 cells incubated with the indicated treatments for 3 h. The full-length blots were presented in Supplementary Figure 2. (d) The expressions of CRT were detected by flow cytometry after CT26 cells incubated with the indicated treatments for 24 h. The results were represented the mean ± S.D. of three independent experiments. *P < 0.05