Fig. 3

SOP inhibits migration and invasion of gastric cancer cells. a AGS cells were treated with or without 3 μM SOP for 24 h and then transwell assay was used to evaluate cellar migration and invasion. b SGC7901 cells were treated with or without 3 μM SOP for 24 h and then transwell assay was used to evaluate cellar migration and invasion. c AGS cells were treated with 5 ng/ml TGF-β alone or in combination with 3 μM SOP for indicated hours, the expression of EMT-related proteins was determined by western blot. Full-length blots/gels are presented in Supplementary Figure S5. E-cadherin expression in (c) was further determined by immunofluorescence (d) and flowcytometry (e) analysis. The results are representatives of at least 3 independent experiments. Data were presented as mean ± SD. ***P < 0.0001. Abbreviation: SOP, Sophoridine