Fig. 4

RUNX2 is a putative target of miR-130a-5p. a the binding sites of miR-130a-5p with RUNX2 predicted by bioinformatics; b the mRNA expression of RUNX2 in A549 and SK-MES-1 cells in response to miR-130a-5p mimic/inhibitor or their controls determined by RT-qPCR (one-way ANOVA, *p < 0.05); c the RUNX2 protein expression in A549 and SK-MES-1 cells in response to miR-130a-5p mimic/inhibitor or their controls evaluated by western blot analysis (one-way ANOVA, *p < 0.05); d the binding relationship between miR-130a-5p and RUNX2 confirmed by the dual-luciferase reporter gene experiment (two-way ANOVA, *p < 0.05). The data are displayed in the form of mean ± SD from three independent experiments