Fig. 6

R269C-ER exhibits differential effect on gene expression in pancreatic cancer cells compared to breast cancer MCF-7 and COLO-357 cells were seeded in phenol red depleted medium with charcoal stripped serum. Cells then were transfected with either WT-ER or R269C-ER in triplicates and then treated with vehicle control or E2 (10nM) for 24 h. Total RNA was extracted and RNAseq was performed. a A heatmap of differentially expressed genes, between untreated mutated and WT-ER, in MCF-7 cells was generated. b A Venn-diagram of significantly (p < 0.05) upregulated or downregulated genes in MCF-7 compared to MCF-7 cells treated with E2 is depicted. c Pathway enrichment analysis was conducted in MCF-7 cells treated with E2 (10nM), using Gene Analytics™. Results show that R269C-ER overexpression is associated with different metabolic pathways. d A heatmap of differentially expressed genes, between untreated mutated and WT-ER, in COLO-357 cells was generated e A Venn-diagram of significantly (p < 0.05) upregulated or downregulated genes in COLO-357 compared to COLO-357 treated with E2 is depicted