Fig. 2

OCT4 and SPP1 expression in human various cell lines and OCT4 in cell migration. Gel electrophoresis of PCR products using primer sets specific to human OCT4A, OCT4Bv, SPP1-all, SPP1C, and GAPDH (positive controls). RT-PCR using specific primer sets for detecting OCT4A and OCT4B variants and SPP1 variants, respectively (Table S3). RT+ and RT− indicate treatments with and without reverse transcriptase, respectively. Arrowheads, OCT4Bns bands; and asterisks, OCT4B-splice variants (OCT4B, OCT4B1, and OCT4B2; denoted as OCT4Bv). a Pooled RNA samples from two patients and human lung tissues (Table S2). b–d Representative bright-field images of wound-healing experiments using endometrial and breast cancer cell lines (Ishikawa vs HEC50B; and MCF7 vs MDA-MD-231) acquired at 24- and 48-h post-wounding. Scale bar: 100 μm. e Representative bright-field images of A549 cells transfected with pOCT4-DTA or pOCT4-GFP control plasmids and acquired at 12-h post-wounding. The migration rate is expressed as the percentage of wound-closure area. Data represent the mean ± standard deviation of the closure area at each time point from three independent experiments. *P < 0.05compared with the control. Scale bar: 100 μm. All full-length gels are presented in Supplementary Figs. S5–1-5