Fig. 1

OCT4A- and OCT4B-transcript variants are expressed in various human somatic tissues. a Gel electrophoresis of PCR products derived from primer sets specific for human OCT4A- and OCT4B-transcript variants and GAPDH (loading control). RT+ and RT− indicate treatments with and without treatment of reverse transcriptase, respectively. Detailed information on RNA sources is presented in Table S2. Three independent experiments were performed, and representative images are shown. Arrow, OCT4A; arrowhead: OCT4Bns; bracket: OCT4Bv (B-splice variants other than OCT4Bns). b The size and position of expected bands are indicated in a partially expanded image surrounded by a dashed line (left). Schematic diagram of human OCT4 mRNA variant structures (right). c Expression of human OCT4A- and OCT4B-transcript variants in the pooled sample and in two samples of normal adult lung tissues from different sources. d Semi-quantitative comparison of human OCT4A-expression levels between PA-1 cells and adult tissues (liver, lung, pancreas, and testis). A 10-fold series of dilutions of cDNA from PA-1 cells were used for standard PCR, and a 2-fold series of dilutions of cDNA from human tissue samples were used for comparison of PCR samples. All full-length gels are presented in Supplementary Figs. S4–1-4