Fig. 7
Effect of GT3 and AT on prostate cancer cells in the presence of inhibitor U0126.a, b and c: Prostate cancer cells (a) LNCaP, b PC-3, and (c) non-tumorigenic RWPE-1 cells were grown in 96-well plates. Two plates were used per cell line; plate one was pre-treated with inhibitor U0126 for 1 h, after which, plates one and two were dosed for 24 h with GT3 at 0, 20, 40 and 80 μM. Cell viability was analyzed by MTT or metabolic assays. For quantification spectrophotometric data were calculated as percentages of the value for the untreated cells (100%) ± standard deviations from at least three independent experiments, *p < 0.05, ***p < 0.001. Statistical analysis using ANOVA and Bonferroni test indicates a significant difference between cells treated with and without inhibitor